Nature research paper: SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry
for 5 min at 4 °C; the supernatant was removed. Cells transfected with ORF8 construct and non-transfected control cells were then gently resuspended in 1 ml FACS buffer with a 1:500 dilution of Strep-Tactin DY-488 and rotated at 4 °C for 1 h, protected from light. Cells were then washed twice in 1 ml FACS buffer, resuspended in 1 ml FACS buffer and filtered through a 35-µm mesh into FACS tubes. A BD Influx cell sorter was used to analyse cells.
Dried histone peptides were reconstituted in 0.1% formic acid. A synthetic library of 93 heavy labelled and derivatized peptides containing commonly measured histone PTMsfor endogenous peptides and 100 fmol µlfor each heavy labelled synthetic analyte. For each analysis, 1 µl of sample was injected onto the column for data-independent analysis on a Q-Exactive HF instrument attached to an Ultimate 3000 nano-UPLC system and Nanospray Flex ion source . Using aqueous solution of 0.
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SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry - NatureThe SARS-CoV-2 protein ORF8 functions as a mimic of histone H3 to disrupt host cell epigenetic regulation.
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