A novel tagmentation-based tool specifically predicts genome-wide off-targets of prime editors. NBThighlight
HeLa cells were seeded in a 24-well plate in preparation for transfection. One day after seeding, cells were transfected with an adequate amount of plasmid and 2 μl Lipofectamine 2000 .
1 × 10K562 cells were electroporated with the above-mentioned quantities of plasmid via a Neon transfection system . One day after transfection , antibiotic selection was conducted using puromycin at a concentration of 2 mg/ml. Puromycin selection was continued for 2 weeks [for TAPE-seq and fluorescence-activated cell sorting ], 4 weeks , or 2 days . Genomic DNA was purified with a Blood Genomic DNA Extraction Mini Kit following the manufacturer’s instructions..
France Dernières Nouvelles, France Actualités
Similar News:Vous pouvez également lire des articles d'actualité similaires à celui-ci que nous avons collectés auprès d'autres sources d'information.
T cell characteristics associated with toxicity to immune checkpoint blockade in patients with melanoma - Nature MedicineClonally diverse and activated memory CD4+ Tcells at baseline are associated with the development of severe irAEs in patients with melanoma treated with immune checkpoint inhibitors immunotherapy toxicity AaronNewmanLab aadel_chaudhuri ax_lozano
Lire la suite »
scChIX-seq infers dynamic relationships between histone modifications in single cells - Nature BiotechnologyAnalysis of two histone marks in single cells reveals interplay between modifications.
Lire la suite »
Accurate isoform discovery with IsoQuant using long reads - Nature BiotechnologyIsoQuant predicts novel isoforms from long-read RNA sequencing.
Lire la suite »
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models - Nature BiotechnologyClinical multi-omics data are integrated and analyzed using a generative deep-learning model.
Lire la suite »
Real-time, volumetric imaging of radiation dose delivery deep into the liver during cancer treatment - Nature BiotechnologyReal-time, volumetric imaging of radiation dose delivery deep into the liver during cancer treatment
Lire la suite »