This article explores the microglia and macrophage populations in human glioblastoma tissue and howtheir phenotyping and spatial analysis could be potentially applied to clinical studies.
Sponsored Content by Cerba ResearchMar 16 2023 Glioblastoma mutliforme represents the most dangerous and malignant primary brain tumor.
The use of multiplex immunofluorescence offers a potential solution to this task, with the technical benefit of facilitating the profound phenotyping and analysis of the spatial organization of cells in the tumor microenvironment. Representative images from left to right feature: merged multiplex, macrophage phenotyping targets , M1 HALO® masks, M2 HALO® masks, microglia , and microglia HALO® masks.
It was then possible to perform analysis with HALO® to determine staining concordance between the multiplex slide and simplex slides.Figure A displays an example image of a HISTOPROFILE® Neuro M1/M2 multiplex stained slide: GFAP , TMEM119 , CD68 , CD163 , c-Maf .Figure B features example images that correspond to the multiplex slide for CD68 and the simplex slide . It also shows the corresponding HALO® masks, which confirm the identification of positive cells.
Conclusion The study presented here investigated human GBM tissues using a multiplex panel comprised of GFAP, TMEM119, CD68, c-Maf and CD163, the HISTOPROFILE® Neuro M1/M2. Using multi-spectral acquisition, it was possible to easily phenotype and localize microglia and macrophage populations in the tissues examined.
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