Artists such as Leonardo da Vinci, Caravaggio, and Sandro Botticelli would mix egg yolks into their paints to create a medium called egg tempera.
The study’s authors found the Old Masters might have added egg yolk to their paintings in order to better preserve them against humidity, surface wrinkling, and—ironically—yellowing of the painting. The proteins from the egg yolk helps prevent water uptake from humid areas, which are particularly prevalent in places like Italy where many of the Old Masters worked their craft.
“Adding some proteinaceous material during pigment preparation, resulting in a coating layer, might have solved the problem of unintentional formation of capillary suspensions, resulting in better, more stable paints with higher pigment content,” the paper stated.Bavarian State Painting Collections, Munich
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