Nature research paper: The pupal moulting fluid has evolved social functions in ants
, and the maximum injection time at 80 ms. Relative quantification of metabolite abundances was performed using Skyline Daily v 20.1 with a 2 ppm mass tolerance and a pooled library of metabolite standards to confirm metabolite identity . Metabolite levels were normalized by the mean signal of 8 heavyThe raw data were searched for a targeted list of ~230 polar metabolites and the corresponding peaks were integrated manually using Skyline Daily software.
using one-way repeated-measures ANOVA with auto-scaling normalization and FDR = 0.05. Details of statistical tests are given in the respective figure legends.
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