Study shows SARS-CoV-2 causes DNA damage and elicits an altered DNA damage response NatureCellBio ICGEB LeibnizLIV DNA DNAdamage SARSCoV2 COVID19 coronavirus covid
By Bhavana KunkalikarMar 14 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in Nature Cell Biology, researchers assessed the association between severe acute respiratory syndrome coronavirus 2 infection and deoxyribonucleic acid damage and cellular senescence.
About the study In the present study, researchers demonstrated that SARS-CoV-2 damages DNA and triggers an altered DNA damage response. Furthermore, reverse transcription-quantitative polymerase chain reaction , immunofluorescence, and immunoblotting were employed to determine the amounts of ribonucleotide reductase M2 messenger RNA and protein. To evaluate if CHK1 depletion is enough to recreate the events after SARS-CoV-2 infection, the researchers investigated the effect of CHK1 depletion by RNA interference.
Immunology eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy Infected human pulmonary epithelial Calu-3 cells produced comparable outcomes. Infection of human nasal epithelial primary cells with SARS-CoV-2 confirmed DDR activation, as indicated by pRPAS4/8 and H2AX foci. As evaluated by tail moment, DNA fragmentation was induced in both SARS-CoV-2-infected cell lines relative to control conditions.
Flow cytometry demonstrated that cells knocked down for CHK1 aggregated in S-phase. Pulse labeling with BrdU prior to flow cytometry analysis revealed a greater proportion of BrdU-negative S-phase cells in comparison to control samples. The team also noted that the depletion of CHK1 was sufficient to decrease RRM2 levels and result in DNA damage.
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