Nature research paper: Reversible RNA phosphorylation stabilizes tRNA for cellular thermotolerance
polysulfide solution was added instead of elemental sulfur, and the media were solidified with 1.0% Gelrite . When0medium for construction of the gene knockout strain.For small-scale preparation , archaeal cells were resuspended in 3 ml solution D , 0.5% -lauroylsarcosine sodium salt and 1 mM 2-mercaptoethanol) and mixed with an equal volume of water-saturated phenol and 1/10 volume of 3 M sodium acetate .
The mixture was shaken for 1 h on ice to which 106 ml chloroform/isoamyl alcohol was added, followed by centrifugation at 4,500for 20 min at 4 °C. The supernatant was collected and mixed with 106 ml chloroform/isoamyl alcohol , followed by centrifugation at 4,500for 15 min at 4 °C. The aqueous phase was collected and then subjected to isopropanol precipitation.
France Dernières Nouvelles, France Actualités
Similar News:Vous pouvez également lire des articles d'actualité similaires à celui-ci que nous avons collectés auprès d'autres sources d'information.
RNA binding proteins help T cells pick their weapons before battleScientists at the Babraham Institute have shown that two RNA binding proteins hold the key to a stronger immune response to influenza in mice. Their findings, published today in Nature Communications, reveal that the absence of these proteins changes the potency of T cells that arise at the start on an infection. Further research could lead to implications for therapies that harness the immune system, and for vaccine design.
Lire la suite »
RNA binding proteins help T cells pick their weapons before battleScientists at the Babraham Institute have shown that two RNA binding proteins hold the key to a stronger immune response to influenza in mice. Their findings, published today in Nature Communications, reveal that the absence of these proteins changes the potency of T cells that arise at the start on an infection. Further research could lead to implications for therapies that harness the immune system, and for vaccine design.
Lire la suite »