A research group has developed a new method that further improves the existing CRISPR/Cas technologies: it allows a more precise and seamless introduction of tags into proteins at the gene level. This technology could significantly improve research on proteins in living organisms and opens up new possibilities for medical research.
The research group led by Prof. Markus Affolter at the Biozentrum, University of Basel, has developed a new method that further improves the existing CRISPR/Cas technologies: it allows a more precise and seamless introduction of tags into proteins at the gene level. This technology could significantly improve research on proteins in living organisms and opens up new possibilities for medical research.
Prof. Markus Affolter's team at the Biozentrum, University of Basel, has now developed a new method called SEED/Harvest in the fruit fly . This method combines the CRISPR-Cas9 technique with the Single-Strand Annealing repair pathway, enabling genome-wide changes to be carried out more efficiently and without leaving unwanted scars. The study has been published in Developmental Cell.The SEED/Harvest method proceeds in two steps.
This method is not only significant for genetics and biotechnology."The SEED/Harvest method could also be of interest for medical research, for example, to identify defects caused by disease genes," says Affolter.Gustavo Aguilar, Milena Bauer, M. Alessandra Vigano, Sophie T. Schnider, Lukas Brügger, Carlos Jiménez-Jiménez, Isabel Guerrero, Markus Affolter.
Human Biology Personalized Medicine CRISPR Gene Editing Biology Biochemistry Research Organic Chemistry Biochemistry Forensic Research
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