Nucleophagy delays aging and preserves germline immortality - Nature Aging

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Nucleophagy delays aging and preserves germline immortality - Nature Aging
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Scientists uncover a novel cellular mechanism that regulates aging and fertility FORTH_ITE NatureAging

GFP::LGG-1, the GFP puncta area was measured using the maximum pixel intensity of the Z-stacks. In all quantifications, background subtraction and threshold setting were performed.All animal protocols were approved by the FORTH Ethics Committee and the Directorate of Agricultural and Veterinary Political Region of Crete .

Vibrance Antifade Mounting Medium with DAPI was used to mount coverslips on slides and stain nuclei. Confocal images of fluorescently labeled proteins were captured using a 40× objective lens on an LSM 710 NL multi-photon confocal microscope . Puncta number, size and pixel intensity measurements were performed with ImageJ after background subtraction and threshold setting.

Vibrance Antifade Mounting Medium with DAPI. Fluorescent images were acquired using a 60× objective lens of the EVOS FL Auto 2 Cell Imaging System. Images were processed and cropped using ImageJ.

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