New imaging technique can capture entire planttissues in 3D salkinstitute NaturePlants
root tips were immersed in FAA for 1 h at room temperature. RNase-free water was used throughout the entire protocol. Samples were then dehydrated in a series of 10-min washes once in 70% ethanol, once in 90% ethanol and twice in 100% ethanol, followed by two 10-min washes in 100% methanol, and then were stored in 100% methanol at −20 °C overnight. The next day, samples were rehydrated in a series of 5-min washes in 75% , 50% and 25% methanol in DPBS-T at room temperature.
probes was heated at 90 °C for 5 min and cooled at room temperature. Samples were incubated in hybridization buffer in a 40 °C humidified oven overnight. After hybridization, samples were washed twice in DPBS-TR and once in 4× SSC in DPBS-TR for 30 min at 37 °C and rinsed with DPBS-TR at room temperature. Samples were then incubated in a T4 DNA ligation mixture at room temperature overnight.
probes for UBQ10 were used .The cost of PHYTOMap experiments is approximately US$80 for a 28-gene experiment and US$230 for a 96-gene experiment (Supplementary Fig.
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