An article published in BMCBiology discusses a new method for stable targeting of unperturbed intestinal epithelium during early development. The method could speed up discoveries on intestinal epithelium biology.
Together, this study demonstrates a new microinjection technique that enables robust, reproducible, and stable in vivo targeting of intestinal stem cells in situ in the unperturbed intestinal epithelium. The technique enables researchers to genetically target ISCs and the intestinal epithelium in a physiological in vivo setting. The new technique is capable of transducing all regions of the gastrointestinal tract including stomach, small intestine, and colon.
All animals were housed in centralized facilities at the Fred Hutchinson Cancer Center under the care and supervision of the Comparative Medicine Unit . The animal facility is managed in compliance with the Guide for the Care and Use of Laboratory Animals, 2011. The FHCC is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care, International assured by NIH Office of Animal Welfare and registered as a research facility with the USDA.
Once pulled, the needle tip was snapped off using fine tip forceps at the level where its diameter was30 μm. The needle tip was bevelled at 25° on a fine-grade abrasive plate with regular wetting for 10 min. Afterwards, the needle tip was microscopically checked under × 10 magnification to ensure a clean bevel without deformities.
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The swan genome and transcriptome, it is not all black and white - Genome BiologyBackground The Australian black swan (Cygnus atratus) is an iconic species with contrasting plumage to that of the closely related northern hemisphere white swans. The relative geographic isolation of the black swan may have resulted in a limited immune repertoire and increased susceptibility to infectious diseases, notably infectious diseases from which Australia has been largely shielded. Unlike mallard ducks and the mute swan (Cygnus olor), the black swan is extremely sensitive to highly pathogenic avian influenza. Understanding this susceptibility has been impaired by the absence of any available swan genome and transcriptome information. Results Here, we generate the first chromosome-length black and mute swan genomes annotated with transcriptome data, all using long-read based pipelines generated for vertebrate species. We use these genomes and transcriptomes to show that unlike other wild waterfowl, black swans lack an expanded immune gene repertoire, lack a key viral pattern-recognition receptor in endothelial cells and mount a poorly controlled inflammatory response to highly pathogenic avian influenza. We also implicate genetic differences in SLC45A2 gene in the iconic plumage of the black swan. Conclusion Together, these data suggest that the immune system of the black swan is such that should any avian viral infection become established in its native habitat, the black swan would be in a significant peril.
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High adherence to procalcitonin-guided antibiotic prescribing guideline may reduce antibiotic use in hospitalsA study published in the journal BMC Infectious Diseases claims that high adherence to procalcitonin-guided antibiotic prescribing guidelines is necessary to reduce antibiotic use in patients with suspected lower respiratory tract infections.
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How did UK social distancing restrictions affect the lives of women experiencing intimate partner violence during the COVID-19 pandemic? A qualitative exploration of survivor views - BMC Public HealthBackground Increased numbers of domestic abuse cases were reported at the start of the COVID-19 pandemic. Many people experiencing abuse faced barriers to seeking support with service closures affecting the sector. Available evidence suggests women are overrepresented in the reported cases of intimate partner violence (IPV) and we aimed to learn more about how their lives were impacted by social distancing restrictions. Methods We conducted an online qualitative interview study, using reflexive thematic analysis. Interviews were conducted between April 2021 and March 2022. 18 women in the UK with past experiences of IPV provided informed consent and participated in this study. Results During the analysis, we identified five themes relating to the impact of lockdown restrictions on participants’ lives, including: (1) Lockdown meant being confined to a place where abuse was escalating, (2) Barriers to accessing support, including “cancelled” services and missed opportunities to intervene during interactions in lockdown with frontline workers. (3) Increased feelings of fear, isolation, and loss of control, particularly during the early stages of the pandemic from the combination of abuse and pandemic-related changes to daily life. (4) Some forms of support were more accessible during the pandemic, such as provision of online psychological support and social groups. Participants also accessed new forms of support for the first time during the pandemic, in some cases sparked by posts and content on social media about abuse awareness. (5) For some, psychosocial wellbeing transformed during the pandemic, with several participants using the word “freedom” when reflecting on their experience of simultaneously escaping abuse and living through the COVID-19 pandemic. Conclusions In this study, we explored the views of female survivors of IPV in the UK during the COVID-19 pandemic. Our results highlight the importance of combined public awareness campaigns and community interve
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Labour calls on SNP and Tory Governments to publish trans law legal adviceScottish Labour’s social justice chief has called for the UK and Scottish Governments to publish their legal advice on Holyrood’s controversial gender reforms
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SNP minister refuses to intervene after trans woman rapist sent to Cornton ValeKeith Brown was called to answer an urgent question on the case of Isla Bryson, who was found guilty of two counts of rape
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