Studying partial remission of type 1 diabetes in children: New biomarkers and the immunoregulatory role of microRNA
Wild-type NOD mice were originally obtained from the Jackson Laboratory and then kept in the Animal Facility of the Centre de Medicina Comparativa i Bioimatge de Catalunya under specific pathogen-free conditions. The colony was subjected to a 12 h dark/12 h light cycle and controlled temperatures between 19–23 °C with 40–60% humidity and fed with ad libitum access to acidic water at pH 5 and irradiated Teklad Global 18% Protein Rodent Diet .
, which is homologous between mice and man. Here, we used an antisense oligonucleotide, called miRCURY LNAmiRNA inhibitor, for miR-30d-5p and a miRNA inhibitor negative control A . Thus, three groups composed of six prediabetic NOD mice of 9 weeks of age each were respectively treated with miRNA-inhibitor , miRNA-inhibitor control , or saline . Mice received four i.p. doses every three days at 9 mg/kg in 200 µL saline solution .
]: 0, intact islets/no insulitis; 1, peri-islet infiltrates; 2, 75% islet infiltration or complete islet destruction. A double-blind analysis was performed by independent observers.To determine changes in the percentage of immune cell subpopulations induced by miRNA blockade, the spleen and PLN of all mice were immunophenotyped by flow cytometry.
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