Genome editing techniques based on bacterial-artificial-chromosome in herpesvirus research Chromosome Genome GenomeEditing Research Cas9 CRISPR herpesvirus reversegenetics Micro_MDPI
Review: Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research. Image Credit: Panuwach / Shutterstock
While the RecA-mediated homologous recombination approach for editing herpesvirus genomes present in eukaryotic cells is more effective than conventional methods, it has considerable limitations. Due to the existence of repetitive sequences in the genomes of the herpes virus, one key issue is that RecA expression can generate instability in the herpes virus's BAC clone, which can result in viral genome mutations.
Immunology eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy Schematic diagram of base editing method: dCas9 mediates targeting without DSB formation; cytosine deaminase converts C to U; single-base precise editing is complete with the replication of DNA.
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Childhood adiposity and novel subtypes of adult-onset diabetes: a Mendelian randomisation and genome-wide genetic correlation study - DiabetologiaAims/hypothesis We investigated whether the impacts of childhood adiposity on adult-onset diabetes differ across proposed diabetes subtypes using a Mendelian randomisation (MR) design. Methods We performed MR analysis using data from European genome-wide association studies of childhood adiposity, latent autoimmune diabetes in adults (LADA, proxy for severe autoimmune diabetes), severe insulin-deficient diabetes (SIDD), severe insulin-resistant diabetes (SIRD), mild obesity-related diabetes (MOD) and mild age-related diabetes (MARD). Results Higher levels of childhood adiposity had positive genetically predicted effects on LADA (OR 1.62, 95% CI 1.05, 2.52), SIDD (OR 2.11, 95% CI 1.18, 3.80), SIRD (OR 2.76, 95% CI 1.60, 4.75) and MOD (OR 7.30, 95% CI 4.17, 12.78), but not MARD (OR 1.06, 95% CI 0.70, 1.60). Conclusions/interpretation Childhood adiposity is a risk factor not only for adult-onset diabetes primarily characterised by obesity or insulin resistance, but also for subtypes primarily characterised by insulin deficiency or autoimmunity. These findings emphasise the importance of preventing childhood obesity. Graphical abstract
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EY China staff encouraged to wear Communist party badges\n\t\t\tKeep abreast of significant corporate, financial and political developments around the world.\n\t\t\tStay informed and spot emerging risks and opportunities with independent global reporting, expert\n\t\t\tcommentary and analysis you can trust.\n\t\t
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Woodside says Europe and China hold keys for energy market this year\n\t\t\tExpert insights, analysis and smart data help you cut through the noise to spot trends,\n\t\t\trisks and opportunities.\n\t\t\n\t\tJoin over 300,000 Finance professionals who already subscribe to the FT.
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China lithium: eco probe enables CATL to milk the situation\n\t\t\tExpert insights, analysis and smart data help you cut through the noise to spot trends,\n\t\t\trisks and opportunities.\n\t\t\n\t\tJoin over 300,000 Finance professionals who already subscribe to the FT.
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Congress to examine operations of US companies in China\n\t\t\tKeep abreast of significant corporate, financial and political developments around the world.\n\t\t\tStay informed and spot emerging risks and opportunities with independent global reporting, expert\n\t\t\tcommentary and analysis you can trust.\n\t\t
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Mine collapse in northern China makes trucks look like ants as 48 remain missingA devastating mine collapse in China's Inner Mongolia region killed six and left 48 missing - with footage from the disaster making trucks look like ants in a staggering display.
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