Nature research paper: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining
. Comparison of observed eIF5B association times in tethered mRNA versus tethered 48S PIC experimental setups at 30 °C. The pre-equilibration of the 48S PIC prior to tethering allowed the slow upstream step to proceed, which enabled rapid and concentration-dependent eIF5B association. Fits of the observed association times are represented by the lines, which yielded the indicated rates . In one experiment , the 48S PIC was formed and pre-equilibrated in the absence of eIF5, and then 2.
Extended Data Fig. 5 eIF1A and eIF5B reside simultaneously on initiation complexes when the 60S subunit joins.. Schematic of single-molecule experiments that examined eIF1A and eIF5B dynamics on the 48S initiation complex at equilibrium. Pre-formed 48S initiation complexes on β-globin mRNA were tethered at equilibrium within ZMWs in the presence of 1 mM ATP and GTP.
Extended Data Fig. 6 Fourier Shell Correlation curves, local resolution, particle distribution plot and representative cryo-EM density examples.. Fourier Shell Correlation curves computed for independently refined half-maps of the final subgroup of particles before masking and after masking . The red curve corresponds to FSC curve of phase-randomized structure factors beyond 7Å.. Model versus map FSC .
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