Nature research paper: Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly
, In an odd step of CONEXER, recipient cells with a +1/−1 double selection cassette in their genome ; −1,) and a tetracycline resistance ) conferring plasmid encoding arabinose inducible lambda red components and Cas9 are mixed with donor cells and spotted on an agar plate. Donor cells contain an odd BAC and a non-transferable F’ plasmid. During incubation on the plate the BAC is conjugated from donor to recipient cells.
, In an even step of CONEXER, recipient cells with a +2/−2 double selection cassette in their genome ; −2, and a tetracycline resistance ) conferring plasmid encoding arabinose inducible lambda red components and Cas9 are mixed with donor cells and spotted on an agar plate. Donor cells contain an odd BAC and a non-transferable F’ plasmid. During incubation on the plate the BAC is conjugated from donor to recipient cells.
France Dernières Nouvelles, France Actualités
Similar News:Vous pouvez également lire des articles d'actualité similaires à celui-ci que nous avons collectés auprès d'autres sources d'information.
Continuous carbon capture in an electrochemical solid-electrolyte reactor - NatureBy combining O2/H2O redox electrolysis with a modular solid-electrolyte reactor, a design for continuous electrochemical carbon capture showing high capture rates, high Faradaic efficiencies and low energy consumption is demonstrated.
Lire la suite »
Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering - NatureThree-dimensional imaging using a frequency–angular resolving and frequency-modulated continuous-wave LiDAR enabled by a chip-scale acousto-optic beam-steering device has been demonstrated, with the simplicity and low cost of the device making this a promising technology.
Lire la suite »
Biden using CPAP machine to deal with sleep apnea, White House saysPres. Biden's sleep apnea was first disclosed in 2008 and his 2019 medical record revealed he had had several sinus and nasal passage surgeries.
Lire la suite »
Ancient-DNA researcher fired for ‘serious misconduct’ lands new roleFormer co-workers have expressed shock that Charles Sturt University in southeastern Australia has appointed Alan Cooper to its faculty
Lire la suite »
A model of the post-implantation human embryo derived from pluripotent stem cells - NatureNature research paper: A model of the post-implantation human embryo derived from pluripotent stem cells
Lire la suite »