Caltech researchers have developed BonFIRE, a cutting-edge microscopy technique that combines fluorescence and vibrational microscopy. This new approach offers unparalleled single-molecule imaging and uses isotopes to create various vibrational colors, providing deep insights into biological molecul
If you imagine yourself peering through a microscope, you probably picture looking at a glass slide with an amoeba, or maybe a human cell, or perhaps even a small insect of some kind.
“With our new microscope, we can now visualize single molecules with vibrational contrast, which is challenging to do with existing technologies,” says Dongkwan Lee, study co-author and chemical engineering graduate student. Lu Wei, assistant professor of chemistry and investigator with the Heritage Medical Research Institute. Credit: CaltechWei says that fluorescence microscopy allows researchers to observe single molecules, but it does not provide rich chemical information. On the other hand, vibrational microscopy provides that rich chemical information but only works when the molecule being imaged is present in large amounts.
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