Antigen-independent activation enhances the efficacy of 4-1BB-costimulated CD22 CAR T cells - Nature Medicine

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Antigen-independent activation enhances the efficacy of 4-1BB-costimulated CD22 CAR T cells - Nature Medicine
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From ASH22 speaker NathanSinghLab: A bedside-to-bench analysis identifies scFv linker length as an important component of CAR structure and suggests that tonic signaling can be beneficial for 4-1BB-based CARTcells function

We thank F. Chen and N. Koterba for technical assistance with the cytokine quantification assays and J. Schug for assistance with the RNA sequencing. The pediatric trial was supported by the CHOP Immunotherapy Frontier Program, in addition to support from the Emily Whitehead Foundation, V Foundation and Curing Kids Cancer .

These authors contributed equally: Nathan Singh, Noelle V. Frey, Boris Engels, Shannon L. Maude, Saar Gill, Marco Ruella.Division of Hematology and Oncology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USACenter for Cellular Immunotherapies, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA

Nathan Singh, Olga Shestova, Pranali Ravikumar, Katherine D. Cummins, Yong Gu Lee, Raymone Pajarillo, Inkook Chun, Simon F. Lacey, Regina M. Young, Carl H. June, Saar Gill & Marco RuellaBoris Engels, Amy Shyu, Steven L. Highfill, Andrew Price, Linlin Zhao, Liaomin Peng, Brian Granda, Melissa Ramones & Jennifer L. BrogdonDavid M. Barrett, Jessica Perazzelli, Stephan A. Grupp & Shannon L.

Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USAPenn Institute for Biomedical Informatics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USADepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USATranslational and Correlative Studies Laboratory, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USADepartment of...

Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School, Newark, NJ, USACenter for Immunity and Inflammation, Rutgers New Jersey Medical School State University of New Jersey, Newark, NJ, USA

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